Rebirth of the God-level Xueba

【Your every click, every favorite, every recommendation, every subscription is an important encouragement and support for Zhiniao Village】

***********************************

【Rebirth God-level Scholar】Creation page:

***********************************

[Rebirth God-level Scholar] Total serial post!

***********************************

[Signing in is the embodiment of honor and strength, I hope you don't forget to click the sign in the upper right corner while watching the update]

***********************************

Yang Rui silently planned for his own project. The assistants around him only saw him reading literature, dazed (reading literature), doing experiments by himself, and didn't know what Yang Rui was doing.

At this point, Yang Rui is already like a normal laboratory leader.

In fact, Yang Rui's work for the past six months has been all about preparations.

First of all, it is the ever-expanding scale of Sinovel Labs. The full name is polymerase chain reaction, which is a molecular biology technique.

In 1984, as long as anything was associated with "molecules", it would be like the "nano" in the 21st century, entering the high-tech field.

High is high cost, and new is new for new instruments.

Since the construction of Sinovel Laboratory, the cost of equipment has exceeded 1.5 million US dollars, which is Yang Rui's dividend for more than two quarters, which is equivalent to spending one-third of Yang Rui's income.

Any rich man who came here would be scared to pee when he saw such expenses.

This is a pure investment, and it is very mysterious whether it can be recovered. Although Yang Rui has already proved himself once, it is still hard to say whether he will win if he is caught in the second scientific research competition.

Fortunately, Yang Rui does not have a boss, which allows him to prepare freely. Otherwise, although Academician Cai of Peking University values ​​him so much, if Yang Rui says he wants to spend 1 million US dollars to buy equipment, that is out of the question.

In the scientific research world, those with low status must do projects that require few resources. Otherwise, they will be overwhelmed. Without the life of the princess, don't expect your tutor and principal to treat you like a father. The terminal illness of the princess disease, Even if it can't be cured, it is still necessary to control it as much as possible and improve the quality of life a little.

In addition to the soft and hard conditions of the Huarui laboratory, Yang Rui went to Tengchong and made high-temperature polymerases, all of which were preparations for the project.

But he didn't need to tell anyone about his preparations.

Even Huang Mao, who made the high-temperature-resistant polymerase himself, didn't know what the high-temperature-resistant polymerase was used for.

This is a situation often encountered by people engaged in research. Anyway, if it is done, it is considered a result.

Also because he was not in a hurry to use it, Yang Qiu asked Huang Mao to publish the paper later, and he agreed without hesitation.

And down to the high temperature resistant polymerase, it is serious.

It can be complicated or it can be simple. The complicated one is the easiest for the user, the so-called "automatic". Students 20 years later will almost use this kind of machine. Put the material in, press two buttons on the machine, and then wait for the Just spit things out.

Simple is complicated for users, every cycle needs to add polymerase,

The reason for adding polymerase is that each cycle has a denaturation process, through high temperature means, the double strands of DNA are untied, which is a prerequisite for replication.

Before the high-temperature-resistant polymerase was found, the general polymerase could only withstand the temperature of 37 degrees, and the DNA unzipped the double strand, and the polymerase was finished.

Normal DNA is intertwined by two strands. The process of duplicating the two strands with polymerase and putting them together again is the process of duplicating DNA.

In this case, DNA is like a girl covered in white phosphorus and wearing a safety suit.

The polymerase responsible for replication is like a stripped man.

The process of copying DNA requires mating.

And if a man wants to mate, he must first tear off his safety suit.

The safety suit was torn off, and the white phosphorus with a low ignition point burned instantly, and the man with a low heat resistance level also burned to death, and another man needed to be added later, because the white phosphorus had been burned out, and this time the replication was successful.

However, this is only the first set of loops.

When the mated man enters the second cycle, he is apparently about to be burned to death by the new flaming chastity pants, so a third man is added.

There are disadvantages to continuously adding men. First of all, the corpses are difficult to handle, and the appearance and smell are not good, which will affect the subsequent mating. If there are too many, they will even occupy space.

Therefore, the best way is to find a man who is resistant to high temperatures, and it will not be affected if the "burning chastity pants" appear repeatedly.

For a practical product, this technology has practical value only if you find a man with high temperature resistance.

Murris's suffered a disadvantage in this regard.

Yang Ruiguang can save half a year by avoiding this, but he also has time wasting.

He didn't have the right gene fragment in hand.

To be precise, it was Yang Rui who did not have the sequence analysis of the gene fragment.

Human genome sequencing refers to the sequence analysis of human gene fragments one by one.

Because in order to copy the gene fragment, you must first know what is in the gene fragment, that is to say, you need to know all the information about this girl in safety clothing. Has she ever learned judo? how tall? How strong is it?

After the sequence analysis of the gene fragment is determined, the primer can be determined, and then the appropriate polymerase can be dispatched for replication. For example, if you meet a judo girl, you can send Putin instead of Abe. Although the latter has something to do with judo, it is not appropriate. Compatibility, or to be strangled to death without hesitation.

When Murris was doing it, he was in a large company, and he was doing the job of cloning gene fragments himself, so naturally there were analyzes of various gene fragments.

Yang Rui does not have this condition, even in China, so Yang Rui has to find suitable genes in published papers.

This job is easier said than done, mainly because I am afraid of encountering fraudulent papers, or stupid papers written by stupid people.

The so-called don’t be afraid of 10,000, just in case, there are many such things as false papers or wrong papers in China, not in the United States, and neighboring Japan is also exposed every year.

If it is an ordinary paper, Yang Rui will not delve into the authenticity of other people's papers, as long as it is almost usable.

But it is a project worth 3 billion US dollars. Once it succeeds, there will be many pharmaceutical companies and biotechnology companies staring at it. Yang Rui can't let his thesis be based on an unstable paper.

It is not a paper made by a laboratory that is closely related to itself. If there is no verification, there will be any problems at that time. For the $3 billion project, it will be a gap that attracts flies.

If it is a gene fragment analyzed by his company, Yang Rui can also review the experimental process. If it is a gene fragment analyzed in China, Yang Rui can also ask Academician Cai-the other party is willing to show it to the best, and if he is not willing, he can also have a basic judgment.

However, Sinovel Labs has no such reserves.

Then you have to repeat the experiment yourself.

However, the work of gene sequence analysis can be handed over to the "Ion Channel Laboratory" of Peking University.

Because according to Yang Rui's design, genetic analysis was originally one of the main goals of the official laboratory.

The future "Human Genome Project" is a huge project costing hundreds of billions of dollars. Moreover, the gene sequences obtained by the Human Genome Project are all public and not profitable.

No matter how wealthy Yang Rui is, he can only pretend to be innocent in front of this kind of plan.

Of course, genes other than the "Human Genome Project" are still available for profit, such as the gene of Tyrannosaurus rex, you can find something to study, and no one cares about you.

But in any case, these are future plans. For now, repeating the sequence of several gene fragments is purely basic research, and it is also a good way to train troops by handing it over to the "ion channel laboratory".

After carefully selecting several plasmid fragments, Yang Rui immediately started several small projects for cloning target fragment sequences in the ion channel laboratory.

Newcomers Su Xiankai and Fan Zhenlong were eager to prove themselves, so it was no surprise that they took over the job.

Xu Zhengping was a little surprised that Yang Rui repeated experiments so much, but he had a lot of work under him, and he couldn't care about others.

The only problem is that there are not enough experimental dogs.

After working for more than a day, Su Xiankai couldn't help himself, found Yang Rui, and said, "Professor Xu has his students to help, and the laboratory's public assistants, He Quangui and Zhao Pingchuan, don't come every day, and they have some regular jobs when they come. , I need at least one dedicated assistant to do the experiment.”

"This is what it should be. I'll arrange someone to help you first. You can make a report. I'll go to Professor Cai to ask for someone." Yang Rui's laboratory is considered to be in operation, and the two researchers who are currently on the staff are in place, and they will arrive soon. Time to recruit lab dogs.

"Me too." Fan Zhenlong raised his hand at the other end of the laboratory.

"One person and one full-time assistant, I know." Although Yang Rui brought the establishment of the two to Peking University, it will take a long time for the school to arrange teaching positions for them. During this period, they naturally have no way to help themselves It can only be arranged by Yang Rui to find the experimental dog.

However, seeing his own independent laboratory get up, Yang Rui also had a different idea.

He specially asked the two to make a report, found a time when Academician Cai was in the office, rushed over, handed in the report, and said: "I used to do research on coenzyme Q10, with the help of Zeneca, and Hedong University in Hedong Province has set up a joint laboratory called Jielikang Hedong University Laboratory. There are many students in the laboratory, and they are all carefully selected from the Department of Biology of Hedong University. Many people have done it. After two or three years of experiments, I thought, can I find two people from the laboratory of Zenecon Hedong University and transfer them to my ion channel laboratory."

What Yang Rui had to do was to transfer Yao Yue over.

Academician Cai didn't know why, and asked strangely: "Can they agree to transfer people from Hedong University? Teacher or student?"

"I want two students who have been doing experiments for a long time, so that they can get started when they come here, and they don't need to train again. The students who can do experiments in our school have their own choices. You have to choose carefully... Another one, I think Fan Zhenlong and Su Xiankai are both transferred from other schools, newcomers, senior students may not be willing to cooperate with them, I am familiar with the students in the laboratory of Zenecon Hedong University." Yang Rui There are plenty of reasons.

Academician Cai pondered and said: "There was no such precedent before."

Yang Rui smiled: "It's only been a few years since the resumption of the college entrance examination. You have a precedent for doing it. Who dares to make irresponsible remarks. Isn't there that exchange student abroad? Let's exchange two people in one direction, and Hedong University should not object. .”

It is a little troublesome to talk about this matter. Academician Cai is unlikely to agree to it when an ordinary teacher brings it up. However, the status of "Ion Channel Laboratory" in Academician Cai's mind does not stop there. The cooperation problem raised by Yang Rui made Academician Cai a little bit more concerned.

Today's college students are still very arrogant, students from Peking University! Naturally, they are even more arrogant. Fan Zhenlong and Su Xiankai were selected and transferred by Yang Rui. A Peking University student might have delayed the progress of the experiment.

The harmony of the laboratory cannot be seen or touched, but it cannot be ignored.

Thinking of this, Academician Cai asked, "How do you want to operate?"

"Can I ask Zeneca to come forward? Send two students from Zeneca Hedong Laboratory to Peking University to exchange for one to two years. There is no need to change their student status. Let's make arrangements here and treat them like our own students. , It’s time to go to class, to hold a meeting, to focus on the laboratory side, and let Zeneca give a little tuition and miscellaneous fees..."

"What if you're not satisfied?"

"Then just call back."

"Well, we must obtain the consent of the students and their parents, and write a written consent."

"No problem, come to Peking University, there is nothing not to like." Yang Rui said Academician Cai stroked his beard and laughed.

...