I Just Want to Be a Quiet Top Student

Five experimenters entered the observation phase.

The five groups of rats were numbered as Han Meng group, Cui Hualin group, Tang Yaxing group, Du Yuan group and Wu Junqiang group, with half male and half female.

The miracle did not happen for a while, but the tragedy happened soon.

After the anesthesia effect of Du Yuan's rats ended, they died one after another within a day.

The experimental record is:

Rat No. 1 in Du Yuan's group died at 12:42 from intracranial hemorrhage.

Rat No. 2 in Du Yuan's group died at 12:43 from intracranial hemorrhage.

...

Rat No. 10 in Du Yuan's group died at 12:51 from intracranial hemorrhage.

To be precise, the 10 experimental mice in Du Yuan's group died one after another within ten minutes, with an average of one dying per minute.

The cause of death was the same, all of them died of intracranial hemorrhage.

"Hey, I failed." Du Yuan was very annoyed, his experimental plan proved to be a complete failure, and ten of his friends died suddenly and tragically in the laboratory before they could take the IQ test.

Du Yuan could only be annoyed for a minute. He had to race against time to dissect the 10 dead experimental mice. The main work content was to dissect and analyze the brain tissue of the experimental mice, sum up experience and lessons, and accumulate valuable data for the next experimental design.

There are real risks in conducting gene editing experiments on living organisms.

Du Yuan just modified a gene of the experimental mice, which led to the annihilation of his experimental mice in a very short period of time.

"Brother, don't be discouraged, failure is the mother of success." Han Meng comforted Du Yuan, and he said again: "You should sort out the analysis report of your experimental data as soon as possible, and give your conclusion, is it homologous recombination? Is there a problem with the editing method, or is there a problem with your path selection based on this method? Now we can only adopt the method of elimination, trial and error one by one, until we find the relatively correct method and specific path.”

While Du Yuan was summing up the experience and lessons, the other four experimenters continued to observe the rats in their group.

Not long after, Shen Qi joined the ranks of observers. He noticed that the rats in Cui Hualin's group became very manic. The rats in this group bit anyone they could bite. If they could not bite anyone, they would bite the same kind. metal net.

"Squeak!"

The rats in Cui Hualin's group bared their teeth and looked hideous. Now they don't like fresh fruits and vegetables, they only want to bite live things.

"Hey, I failed." Cui Hualin sighed. His little friend was so crazy after gene editing that he couldn't do an IQ test at all. The craziest rat actually bit off its own front foot.

The smell of blood made the rat even more frantic. It slammed into the metal net, trying to break through the cage and bite the living things outside the cage.

Shen Qi patted Cui Hualin's shoulder: "Extract the living cells, preserve the brain tissue, and then execute him."

"Director Shen, why don't we observe for a while longer?" Cui Hualin was unwilling to fail like this, he still had a glimmer of hope.

Shen Qi shook his head: "There is no need to continue to observe, and execute all the experimental mice in your group. I want to see their original data. As I expected, the modification of their SHANK3 gene affected their neurons. This effect Irreversible, they may become even crazier."

"Okay..." Cui Hualin executed his little friend with tears in his eyes.

Before execution, Cui Hualin used atomized anesthesia to stun his little partner, extracted living cells, and then executed by dislocation, dissected and preserved various organs and brain tissues.

There is no good news at all, and bad news comes one after another.

The rats in Tang Yaxing's group did not show crazy aggression, but they became extremely autistic. They curled up in the corner of the cage motionless, like a furry mouse.

"Your group of autistic experimental mice can't carry out follow-up experiments. Extract living cells, preserve brain tissue, and then execute them. I want to see their original data." Shen Qi gave an order.

"It's a pity, it's a pity." Tang Yaxing shook his head and sighed, then executed his little friend.

At present, one group of rats died due to their own reasons, and the other two groups of rats with abnormal behavior were killed by the experimenter.

The progress of human science is inseparable from the contributions made by these experimental animals.

Scientific research continues, and rats continue to contribute to human scientific progress throughout their short lives.

After observation and various physical examinations, the experimental mice in Han Meng's group and Wu Junqiang's group were temporarily normal in terms of physical indicators and animal behavior, and could be tested in the T maze.

I don't know whether it's good news or bad news, but the T-maze test scores of the experimental rats in Wu Junqiang's group remained the same as before.

This group of rats encountered a T-shaped junction in the T-maze, and their choice was always to go to the right.

Most rodents have a one-way preference, and the Wistar rats selected by Shen Qi's team prefer to go to the right.

The T-maze route designed by Wu Junqiang is eighteen right and one left.

Wistar rats can go out of the maze by going to the right 18 times in a row, and then go to the left once, so as to obtain the food placed at the exit of the maze.

The spatial learning ability of the rats in Wu Junqiang's group did not become stronger, they were quite stubborn, and they always turned to the right and never to the left.

In other words, the IQ of the experimental mice in Wu Junqiang's group that had been edited by the SHANK3 gene remained unchanged and remained at an ordinary level.

"The experimental rats in Wu Junqiang's group are worthy of further research." Shen Qi gave the above instruction.

"Okay, I understand!" Wu Junqiang rubbed his hands excitedly. His little friend will not be executed for the time being. His experimental plan has not been proved to be the most suitable one, but it has not been completely rejected.

The five experimenters personally selected their rats, took care of them, and fed them by themselves. They had more or less affection for their little friends.

A moment's life is also life, if they can give the little friends a way to survive, even if they live a few more days, the experimenters are not willing to kill them immediately with their own hands.

Thirty rats were killed, and ten rats were kept. Currently, the rats in the Han Meng group are in the best condition.

Han Meng designed a brand new T-maze route, and his first three experimental mice achieved better results in this maze than they did before receiving SHANK3 gene editing.

"Han Meng's group of experimental rats is very promising." Shen Qi finally waited for a seemingly good piece of good news.

"Brother Meng, this group of rats has improved their learning ability!" Other experimenters gathered around.

Rat No. 4 of Han Meng's group entered the T maze, and it was a female rat.

It has been verified by scientists that female rats of the Wistar rat category are smarter than male rats.

Why female mice are smarter, the essential reason needs to be further confirmed.

But even for smart Wistar females, their rightward preference is still obvious.

Before gene editing, female rat No. 4 in Han Meng's group could only walk out of the all-right T-maze.

In this new T-maze, Han Meng set up two left-handed and right-handed T-junctions.

After the first three female mice failed several times, they remembered the correct route and the wrong route, they walked out of the maze and ate the food.

Female No. 4 performed even better. She only failed twice before getting out of the maze.

The rightward preference of female No. 4 is still the main characteristic, but at the same time, her learning ability has become significantly stronger.

When it realizes that it won't get food by going right, it makes a change and chooses to go left at the same intersection.

Cui Hualin was amazed: "Mouse No. 4 is quite smart! After failing at an intersection once, it can remember that the left direction is correct, and choose to go left in the next attempt! Brother Meng, it seems that your gene editing method is very reliable ah!"