I Just Want to Be a Quiet Top Student

"The most common gene mutations leading to prostate cancer are: AR, 62.7 percent; ETS family, 56.7 percent; TP53, 53.3 percent; and, PTEN 40.7 percent." Shen Qi drew up a preliminary plan for gene therapy for Han Meng.

According to the genetic test results, Han Meng most likely died of prostate cancer.

Then start treatment with genes related to prostate cancer.

In the plan, the pathogenic mechanism of prostate cancer at the genetic level must first be sorted out.

"In addition, BRCA2 gene mutations were found in 13 percent of advanced prostate cancer patients." Cui Hualin assisted Shen Qi in this work.

"In non-androgen receptor-related gene mutations, PI3K signaling pathway accounts for 49 percent, DNA repair system accounts for 19 percent, cell cycle kinase CDKs accounts for 7 percent, and BRAF accounts for 10 percent. Three, the WNT signaling pathway accounts for 5%." Du Yuan's academic attitude is the same as everyone else's, speaking with data, and any experimental design is based on proven or predictable data.

"The next part of the analysis, I am familiar with. Loss of heterozygosity in the PTEN gene reduces the expression level of PTEN protein, leads to genomic instability, and induces high-grade prostate intraepithelial neoplasia. Yes, that's right, that's it. " Tang Yaxing continued.

Wu Junqiang added: "In the case of genome instability, chromosomal rearrangement and recombination are prone to occur, such as TMPRSS2-ERG gene fusion, which will promote the occurrence of prostate cancer. When the homozygous deletion of PTEN gene occurs, the downstream molecule AKT continues Activate and induce the invasion and metastasis of prostate cancer... Hey guys, this is what I wrote for my master's thesis. I asked my supervisor at the time, now that we have figured out the genes involved in thousands of diseases, including cancer Why is gene therapy technology not widely used clinically in terms of pathogenic mechanism? Although there are risks in modifying genes, some people may live for another 20 years after modification. Maybe you can only live for two years. There is a saying in the West that a moment of life is life. Isn’t it a good deed to save lives by using technical means to make a person live a few years or more than ten years? Of course, people who can receive gene therapy , provided that the expensive treatment costs can be afforded.”

Shen Qi asked, "What did your mentor say?"

"My mentor said two words: Negative entropy. Until today, I haven't been able to understand the true meaning of these two words." Wu Junqiang said inexplicably.

"It's a pity, Professor Hu passed away at a young age." Shen Qi sighed sadly, feeling like Boya's child.

Wu Junqiang's mentor, Professor Hu, passed away in his fifties. The real cause of death is unknown, and his family has been looking for clues.

"Director Shen, what is the true meaning of negative entropy? I checked the literature and found that the concept of negative entropy was first proposed by Schrödinger. Schrödinger was a physicist, so why did my mentor quote a physicist's idea? What about the concept? What is the relationship between negentropy and biology? I really can’t figure it out, so I’d like to ask Director Shen to clarify.” Wu Junqiang asked Shen Qi, the two-time Nobel Prize winner, and he realized that Shen Qi and His supervisors seem to share the same academic ideas, but this idea is relatively advanced, so advanced that even Wu Junqiang, a doctoral student who has studied and worked in a famous biological laboratory, can hardly get the Key-point.

Shen Qi said: "Erwin Schrödinger's first career was a physicist, and he also had a second career and a third career. Schrödinger was an all-rounder, and he was proficient in more than just physics. The question you asked Xiao Wu , involves a fairly complicated set of theoretical systems, which is a level deeper than genomics, and even I know one thing but not the other. So let's not discuss Schrödinger's whimsy for the time being, let's go back to the topic, Our protagonist at this stage is Han Meng."

Han Meng clasped his fists and said, "I have to worry about Director Lao Shen and all the brothers."

The pathogenic mechanism of Han Meng's gene therapy has been sorted out clearly.

Next is the detailed discussion of technical operation.

Through gene sequencing, it is first predicted that a seemingly healthy person may suffer from a certain disease, or a certain mutation is detected in someone's DNA, and then the exogenous normal gene is introduced into the specific gene locus of the chromosome in the target body cell, and replaced with a healthy gene Abnormal genes reorganize DNA to achieve the purpose of curing diseases.

There are two ways in terms of technical operation: one is to take out the cells from the patient, introduce the missing DNA into the cells, and then transplant the treated cells back into the patient. It's called in vitro gene therapy.

The second is to inject healthy genes directly into the patient's organs, which is called in vivo gene therapy.

Shen Qi's setting is in vivo gene therapy.

The technical outline is to use microrobots to manipulate microneedles and inject normal foreign genes directly into Han Meng's defective cells.

"Then Han Meng, do you agree with our plan?" Shen Qi asked.

"I agree." Han Meng rolled up his sleeves, and took the pen and document Shen Qi handed him.

This document is the "Research Experiment Volunteer Agreement".

The main clauses in the agreement are: I understand the content of the experiment, voluntarily participate in this experiment, and bear all risks and possible consequences, including but not limited to new mutations, disappearance of vital signs and other consequences.

Attachments: "Han Meng's Current Gene Sequencing Results", "Han Meng's Gene Sequence Prediction After Gene Therapy (Case)"

The drafting of the "Agreement on Volunteers for Scientific Research Experiments" is relatively rigorous, and the rigor is reflected in the text description and attachments.

After Han Meng received gene therapy, his body and appearance will not change.

But biologically, he will become someone else.

For example, if the current Han Meng has a criminal record and filed a record with the Public Security Bureau, the Public Security Bureau will extract his DNA sample data and store it in a computer for future reference.

Then Han Meng after receiving gene therapy, his DNA will not match with his DNA filed in the Public Security Bureau.

The achievements and crimes of Han Meng in the past will become history in biology.

The new Han Meng will start a new life.

Therefore, Shen Qi attached "Han Meng's Current Gene Sequencing Result" and "Han Meng's Gene Sequence Prediction After Gene Therapy (Case)" and asked Han Meng to sign and pledge.

Han Meng has no criminal record, he has not committed any crimes.

He is willing to help others. He helps the grandmother cross the road, donates the books he has finished studying to the Hope School, plants a forest on Alipay, and adopts a hen from the Yunnan-Guizhou Plateau.

From a moral point of view, Han Meng is a good person.

Han Meng's highest achievement is that he published three SCI papers as one author during his Ph.D. study in Canada.

In front of Shen Qi, the three SCI works are not worth showing off.

So Han Meng signed and pressed his fingerprints on the signature column of the "Scientific Research Experiment Volunteer Agreement" without complaint or regret.